Systems and methods for the isolation and identification of microorganisms from hydrocarbon deposits
AssigneeLUCA Technologies, Inc.
InventorGary Vanzin
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Abstract
The present invention provides systems and methods to isolate microorganisms and optionally their genetic material. The isolation of microorganisms is conducted under anaerobic or other similar conditions to facilitate the preparation of microorganisms that are anaerobes, whether obligate or facultative, as well as microaerophiles. In an alternative format, the isolation of microorganisms is from a carbonaceous substrate which typically hinders or retards isolation therefrom.
Claims19
A method of eluting or desorbing nucleic acids adsorbed to a carbonaceous substrate, said method comprising
- contacting cells, associated with said substrate and containing said nucleic acids, with a solution containing one or more anions and a zwitterionic detergent to form a mixture, and
- mechanically agitating said mixture to elute said cells from said substrate into said solution.
A method of preparing a microbial community of microorganisms, said method comprising
- obtaining an anaerobic subterranean substrate comprising viable microorganisms;
- and
- maintaining said substrate under anaerobic conditions to form an ex situ microbial community.
Dependencies are inferred from the claim text.