Polypeptides having nucleic acid binding activity
AssigneeApplied Biosystems, LLC
InventorsPatrick Martin, David Simpson
Life of this patent
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Abstract
Polypeptides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided. Methods of promoting the annealing of complementary nucleic acid strands are provided. Methods of increasing the processivity of a DNA polymerase are provided. Methods of enhancing the activity of a nucleic acid modification enzyme are provided. Fusion proteins are provided. Methods of using fusion proteins are provided. Kits are provided.
Claims13
1-24. (canceled)
A fusion protein comprising:
- a polypeptide comprising an amino acid sequence of a Crenarchaeal nucleic acid binding polypeptide or a fragment thereof having nucleic acid binding activity;
- and
- a nucleic acid polymerase.
A fusion protein comprising:
- a polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:1, wherein the polypeptide has nucleic acid binding activity;
- and
- a nucleic acid polymerase.
A fusion protein comprising:
- a polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:4, wherein the polypeptide has nucleic acid binding activity;
- and
- a nucleic acid polymerase.
A fusion protein comprising:
- a polypeptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO:6, wherein the polypeptide has nucleic acid binding activity;
- and
- a nucleic acid polymerase.
A fusion protein comprising an amino acid sequence selected from SEQ ID NOs:23, 24, 26, 27, 34, 35, 37, 38, 40, 41, 43, and 44.
36-69. (canceled)
Dependencies are inferred from the claim text.