Controlled dissolution crosslinked protein in crystals

A controlled dissolution crosslinked lipase crystal produced by a process comprising the step of crosslinking lipase crystals with at least one crosslinking agent in a crosslinking solution, said crosslinking agent being present in said solution at a concentration between about 0.76 mM and 172.3 mM, wherein said controlled dissolution crosslinked lipase crystal is capable of change from insoluble and stable form to soluble and active form upon a change in the environment surrounding said crystal, said change being selected from the group consisting of:

• change in temperature, change in pH, change in chemical composition, change from concentrate to dilute form, change in shear force acting upon the crystal and combinations thereof and wherein said crosslinking agent is dimethyl 3,3′-dithiobispropionimidate.HCl (DTBP);
• dithiobis (succinimidylpropionate) (DSP);
• bismaleimidohexane (BMH);
• bis[sulfosuccinimidyl]suberate (BS);
• 1,5-difluoro-2,4-dinitrobenzene (DFDNB);
• dimethylsuberimidate-2HCl (DMS);
• disuccinimidyl glutarate (DSG);
• disulfosuccinimidyl tartarate (Sulfo-DST);
• 1-ethyl-3-[3-dimethylaminoproplyl]carbodiimide hydrochloride (EDC);
• ethylene glycolbis[sulfo-succinimidylsuccinate] (Sulfo-EGS);
• N-[γ-maleimido-butyryloxy]succinimide ester (GMBS);
• N-hydroxysulfo-succinimidyl-4-azidobenzoate (Sulfo-HSAB);
• sulfosuccinimidyl-6-(α-methyl-α-(2-pyridylthio)toluamido)hexanoate (Sulfo-LC-SMPT);
• Bis-(β-[4-azidosalicylamido]ethyl)disulfide (BASED);
• NHS-PEG-Vinylsulfone (NHS-PEG-VS);
• (Bis[2-(sulfosuccinimidooxycarbonyloxy)-ethyl]sulfone (Sulfo-BSOCOES);
• glutaraldehyde;
• succinaldehyde;
• octanedialdehyde;
• glyoxal;
• or epichlorohydrin.