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US 2006/0223145 A1Published application
Method for producing recombinant human interferon alpha 2b polypeptide in pichia pastoris
OwnerBraj Lohray, Sarvagna Shah +2inventors
InventorsBraj Lohray, Sarvagna Shah, Hemal Pandit, Megha Patel
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Abstract
This invention relates to a process for expression of recombinant Interferon-alfa2b polypeptide in yeast cells and its method of purification and formulation.
Claims21
A process for preparation and purification of recombinant human interferon (hu-IFN) alpha 2b which comprises:
- I. cultivating recombinant Pichia pastoris containing a hu-IFN alpha 2b gene,
- II. culturing said recombinant Pichia pastoris in complex/defined salt culture medium to produce hu-IFN alpha 2b protein, and
- III. purifying recombinant hu-IFN alpha 2b protein from said culture medium.
Dependencies are inferred from the claim text.