Method for mass production of human pollicle stimulating hormone
AssigneeProgen Co., Ltd.
InventorsSe Hwan Yang, Young Chul Sung, Kyu-Heum Na, Sung Hee Lee, Won Bae Kim
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Abstract
The present invention relates to a method for mass-production of human follicle stimulating hormone, more precisely, to an expression vector containing human follicle stimulating hormone gene, a transformant transfected with the expression vector and a method for mass-production of human follicle stimulating hormone by using the same. Human follicle stimulating hormone can be produced largely by using an expression vector and a transformant of the present invention. Therefore the expression vector and the transformant of the present invention can be effectively used for the treatment of infertility.
Claims16
An expression vector to express human follicle stimulating hormone (FSH) comprising a gene coding human FSH, a promoter sequence, a polyadenylation motif sequence and a dihydrofolate reductase (DHFR) gene.
A method for mass-production of human follicle stimulating hormone comprising the following steps of:
- 1) preparing an expression vector containing human FSH gene;
- 2) transfecting host cells with the expression vector of step 1);
- 3) selection of selecting transformants transfected in step 2);
- 4) selecting a recombinant transformant stably producing human FSH from the recombinant transformants selected in the step 3);
- and
- 5) obtaining human FSH from the culture of the recombinant transformant selected in step 4).
Dependencies are inferred from the claim text.