Method and apparatus for counting megakaryocytes
AssigneeSysmex Corporation
InventorsYusuke Mori, Toshihiro Mizukami, Tomohiro Tsuji, Yoshiro Ikeuchi
Life of this patent
Estimating the term…
Abstract
A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.
Claims21
A method for counting megakaryocytes in a specimen, comprising the steps of:
- preparing a measurement sample by lysing erythrocytes in the specimen and staining nucleic acid in the megakaryocytes with a fluorescent dye;
- irradiating cells in the measurement sample with excited light;
- detecting forward scattered light, side scattered light and fluorescence which is emitted from the cells;
- identifying megakaryocytes on the basis of the detected forward scattered light, side scattered light and fluorescence;
- and
- counting the identified megakaryocytes.
An apparatus for counting megakaryocytes, comprising:
- a measurement sample preparing part for preparing a measurement sample by mixing a specimen that includes megakaryocytes, a lysing reagent for lysing erythrocytes, and a nucleic acid fluorescent dye for staining megakaryocytes;
- a light source for irradiating cells in the measurement sample with excited light;
- a first scattered light detector for detecting forward scattered light which is emitted from cells;
- a second scattered light detector for detecting side scattered light which is emitted from cells;
- a fluorescence detector for detecting fluorescence which is emitted from cells;
- and
- an analyzing part for identifying megakaryocytes on the basis of the detected forward scattered light, fluorescence and side scattered light, and counting the identified megakaryocytes.
Dependencies are inferred from the claim text.