Reagent for partially lysing a cell membrane of a red blood cell, a reagent for detecting malaria infected red blood cells, and a sample analyzing method for detecting malaria infected red blood cells

A sample analyzing method for detecting malaria infected red blood cells, comprising:

• (a) preparing a measuring sample by lysing cell membranes of malaria infected red blood cells in a sample such that a malaria parasite is retained in the red blood cell and fluorescent dye, which is preferential for DNA over RNA, passes through the cell membrane, and by staining DNA of the malaria parasite by the fluorescent dye;
• (b) measuring fluorescent light and scattered light from the red blood cells in the measuring sample prepared in the step (a);
• and
• (c) detecting the malaria infected red blood cells based on the fluorescent light and the scattered light measured in the step (b).

A sample analyzing method for detecting malaria infected red blood cells, comprising:

• (a) lysing cell membranes of malaria infected red blood cells in a sample such that a malaria parasite is held in the red blood cell and fluorescent dye, which is preferential for DNA over RNA, passes through the cell membrane;
• (b) staining DNA of the malaria parasite by the fluorescent dye;
• (c) exposing the sample which contains the red blood cells, to which the treatments in steps (a) and (b) have been applied, to irradiated light and obtaining optical information;
• and
• (d) detecting first red blood cells which have single ring form and/or second red blood cells which have two or more ring forms based on the optical information.