Scytalone dehydrogenase gene showing tolerance to agricultural pesticide
AssigneeKumiai Chemical Industry Co., Ltd.
InventorsKoichiro Kaku, Satoshi Watanabe, Kiyoshi Kawai, Tsutomu Shimizu, Kozo Nagayama
Life of this patent
Estimating the term…
Abstract
The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
Claims11
A gene coding for either one of the following proteins (a) or (b):
- (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2;
- or
- (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.
A method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor, comprising the steps of:
- (a) identifying an amino acid in an amino acid sequence of scytalone dehydratase in a subject rice blast fungus, which corresponds to valine at position 75 in the amino acid sequence shown in SEQ ID NO:
- 4;
- and
- (b) assessing sensitivity of the subject rice blast fungus to the scytalone dehydratase inhibitor based on the results of step (a).
A kit for assessing a rice blast fungus resistant to a scytalone dehydratase inhibitor, comprising a pair of primers designed to flank a nucleotide sequence coding for an amino acid corresponding to valine at position 75 in the amino acid sequence shown in SEQ ID NO:
- 4.
A kit for assessing a rice blast fungus resistant to a scytalone dehydratase inhibitor, comprising an oligonucleotide including a nucleotide sequence coding for an amino acid corresponding to valine at position 75 in the amino acid sequence shown in SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.