Doxorubicin immunoassay
AssigneeSaladax Biomedical Inc.
InventorsSalvatore Salamone, Jodi Courtney, Shu He
Life of this patent
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Abstract
Novel conjugates of doxorubicin and novel doxorubicin immunogens derived from the 13 and 14 positions of doxorubicin and antibodies generated by these doxorubicin linked immunogens all of which are useful in immunoassays for the quantification and monitoring of doxorubicin in biological fluids.
Claims58
An immunoassay for detecting doxorubicin in a sample comprising providing a mixture of a) said sample, b) an antibody selectively reactive with doxorubicin and not substantially cross-reactive with doxorubicin aglycone and c) a conjugate of a carrier having either a reactive thiol or amino group with a compound of the formula:
- wherein A is
- Y is an organic spacing group;
- X is a functional group capable of binding to said carrier through said amino or thiol group;
- and
- p is an integer from 0 to 1;
- or a compound of the formula:
- wherein X, Y and p are as above and B is
- or mixtures thereof;
- causing the doxorubicin in the sample and said conjugate in said mixture to bind in said mixture with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of doxorubicin in the sample can be determined.
An antibody which binds selectively to doxorubicin and does not substantially bind to doxorubicin aglycone.
A compound of the formula:
- wherein A is
- Y is an organic spacing group;
- X is a functional group capable of binding to a thiol or amino group;
- and
- p is an integer from 0 to 1.
A compound of the formula:
- wherein Y is an organic spacing group;
- X is a functional group capable of binding to a thiol or an amino group;
- and
- B is —CH2— or
- or mixtures thereof.
A conjugate of a carrier containing a functional thiol or amino group with a compound of the formula:
- wherein A is
- Y is an organic spacing group;
- X is a functional group capable of binding to said thiol or amino group;
- and
- p is an integer from 0 to 1.
A conjugate of a carrier having a thiol or amine group with a compound of the formula:
- wherein B is —CH2— or
- Y is an organic spacing group;
- X is a functional group capable of binding to said thiol or amino group in said carrier;
- and
- p is an integer from 0 to 1.
A kit for determining the presence of doxorubicin in a patient sample comprising reagents in separate containers, one of the reagents being a conjugate of a carrier containing a functional amino or thiol group with a compound selected from the groups consisting of compounds of the formula:
- wherein A is
- Y is an organic spacing group;
- X is a functional group capable of binding to the amino or thiol group in said carrier;
- p is an integer from 0 to 1;
- and
- or a 14-substituted doxorubicin of the formula:
- wherein X, Y and p are as above and B is —CH2— or
- or mixtures thereof;
- and the second container containing an antibody substantially selectively reactive with doxorubicin and not substantially cross-reactive to doxorubicin aglycone.
Dependencies are inferred from the claim text.