Phenethanolamine-derived haptens, immunogens, antibodies and conjugates
AssigneeRandox Laboratories Limited
InventorsRobert McConnell, Stephen Fitzgerald, El Benchikh, Andrew Lowry
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Abstract
The present invention relates to a method for preparing haptens of formulae I and II that are useful in the preparation of immunogens, antibodies and conjugates, for use in competitive immunoassays for the detection of ractopamine, isoxsuprine and ritodrine. The haptens are prepared by reacting a phenylethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E: in which, in formula I, Z1 is a crosslinker and Z2 is H and, in formula II, Z1 is H and Z2 is a crosslinker.
Claims36
A method of preparing a hapten of formula I
- suitable for use in raising an antibody capable of binding with at least one structural epitope of a phenethanolamine, the method comprising the steps of
- preparing a phenethanolamine derivative of the formula D by reacting octopamine hydrochloride with Z1X1, in which Z1 is a crosslinker, the crosslinker being —R—X2, in which R is a bivalent link and X2 is a functional group containing at least one hetero-atom capable of linking the R of the crosslinker with a moiety selected from the group comprising an antigenicity-conferring material and a detectable labeling agent, and X1 is a halide;
- reacting the phenethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E, in which Z2 is H;
- Y is independently H or CH3;
- W is independently H or CH3;
- n is independently 1 or 2;
- and m is independently 0 or 1
(canceled)
A method of preparing a hapten of formula II
- suitable for use in raising an antibody capable of binding with at least one structural epitope of a phenethanolamine, the method comprising the steps of
- preparing a phenylalkylcarbonyl derivative of the formula E by reacting 4-(4-hydroxyphenyl)-2-butanone with Z2X1, in which Z2 is a crosslinker, the crosslinker being —R—X2, in which R is a bivalent link and X2 is a functional group containing at least one hetero-atom capable of linking the R of the crosslinker with a moiety selected from the group comprising an antigenicity-conferring material and a detectable labeling agent, and X1 is a halide;
- reacting a phenethanolamine derivative of formula D, in which Z1 is H, with the phenylalkylcarbonyl derivative of formula E;
- Y is independently H or CH3;
- W is independently H or CH3;
- n is independently 1 or 2;
- and m is independently 0 or 1
5-7. (canceled)
Dependencies are inferred from the claim text.