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OwnerBarry Schweitzer, James Ball +3inventors
InventorsBarry Schweitzer, James Ball, Paul Predki, Fang Zhou, Gregory Michaud
Estimating the term…
The present invention provides human protein arrays that include at least 1000 human proteins. In another embodiment, the present invention provides a method for identifying a substrate of an enzyme, comprising contacting the enzyme with a positionally addressable array comprising at least 100 proteins immobilized on functionalized glass surface, and identifying a protein on the positionally addressable array that is bound and/or modified by the enzyme, wherein a binding or modifying of the protein by the enzyme indicates that the protein is a substrate for the enzyme. In additional embodiments, provided herein are methods for making an array of at least 1000 human proteins under non-denaturing conditions, including human proteins that are difficult to express and/or difficult to isolate in a non-denatured state.
A positionally addressable array comprising 100 human proteins from the proteins listed in Table 9, Table 11, and Table 13, immobilized on a substrate.
19-22. (canceled)
A method for identifying a substrate of an enzyme, comprising contacting the enzyme with a positionally addressable array comprising at least 100 proteins immobilized on a functionalized glass slide, and identifying a protein on the positionally addressable array that is modified by the enzyme, wherein a modifying of the protein by the enzyme indicates that the protein is a substrate for the enzyme.
31-43. (canceled)
A method for making an array of proteins, comprising:
The method of claim 48, wherein the proteins comprise at least 50 transmembrane proteins of Table 16.
The method of claim 49, wherein the proteins comprise at least 25 G-protein coupled receptor proteins of Table 17.
53-61. (canceled)
Dependencies are inferred from the claim text.