Amphiphilic mediated sample preparation for micro-flow cytometry
AssigneeThe Regents of the University of California
InventorsDavid Clague, Elizabeth Wheeler, Abraham Lee
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Abstract
A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.
Claims13
A flow cytometry method for detecting a sample, comprising the steps of:
- attaching a hydrophobic unit to the sample,
- providing an oil and water combination with an interface between said oil and said water,
- placing the sample and said hydrophobic unit in said oil and water combination allowing the sample and said hydrophobic unit to reach said interface, and
- detecting the sample at said interface.
Dependencies are inferred from the claim text.