Back to results
US 2006/0223097 A1Published application
Capturing sequences adjacent to type-IIS restriction sites for genomic library mapping
AssigneeAffymetrix, Inc.
InventorsRonald Sapolsky, Robert Lipshutz, Thomas Gingeras
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic napping, screening and manipulation.
Claims12
A method of identifying sequences in a polynucleotide sequence, comprising:
- first cleaving the polynucleotide sequence with a first type-IIs endonuclease;
- first ligating a first adapter sequence to the polynucleotide sequence cleaved in said first cleaving step, said first adapter having a recognition site for a second type-IIs endonuclease;
- second cleaving the polynucleotide sequence resulting from said first ligating step, with the second type-IIs endonuclease;
- second ligating a second adapter sequence to the polynucleotide sequence cleaved in said second cleaving step;
- and
- determining the sequence of nucleotides of the polynucleotide sequence between the first and second adapter sequences.
3 more elements
Dependencies are inferred from the claim text.