Methods and compositions for producing labeled probe nucleic acids for use in array based comparative genomic hybridization applications
OwnerDouglas Amorese, Diane Ilsleyinventors
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Abstract
Methods and compositions for producing labeled probe nucleic acids from genomic nucleic acid template are provided. In the subject methods, a conserved coding consensus region primer is employed to enzymatically generate a select set of labeled probe nucleic acids corresponding to coding regions of genes from a genomic template via a primer extension protocol. The subject methods find use in a variety of different applications, and are particularly suited for use in the preparation of labeled probe nucleic acids for use in array based comparative genomic hybridization applications. Also provided are kits for use in practicing the subject methods.
Claims10
1-17. (canceled)
An oligonucleotide primer comprising a conserved coding consensus region.
A kit for use for use in comparing the relative copy number of nucleic acid sequences in two or more collections of nucleic acid molecules, said kit comprising:
- (a) an oligonucleotide primer comprising conserved coding consensus region;
- and
- (b) instructions for practicing the method according to claim 1.
Dependencies are inferred from the claim text.