Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof
AssigneeApplera Corporation
InventorsMay Luke, James Devlin, Dov Shiffman, Michele Cargill
Life of this patent
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Abstract
The present invention is based on the discovery of genetic polymorphisms that are associated with coronary heart disease and in particular stenosis and MI and response to drug treatment. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection.
Claims20
A method for identifying an individual who has an altered risk for developing CHD, comprising detecting a single nucleotide polymorphism (SNP) in any one of the nucleotide sequences of SEQ ID NOS:
- 1-67 and 135-504 in said individual's nucleic acids, wherein the SNP is as specified in Table 1 and Table 2, respectively, and the presence of the SNP is correlated with an altered risk for MI in said individual.
An isolated nucleic acid molecule comprising at least 8 contiguous nucleotides wherein one of the nucleotides is a single nucleotide polymorphism (SNP) selected from any one of the nucleotide sequences in SEQ ID NOS:
- 1-67 and 135-504, or a complement thereof, wherein the SNP is as specified in Table 1 and Table 2, respectively.
An isolated nucleic acid molecule that encodes any one of the amino acid sequences in SEQ ID NOS:
- 68-134.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 68-134.
An amplified polynucleotide containing a single nucleotide polymorphism (SNP) selected from any one of the nucleotide sequences of SEQ ID NOS:
- 1-67 and 135-504, or a complement thereof, wherein the amplified polynucleotide is between about 16 and about 1,000 nucleotides in length.
An isolated polynucleotide which specifically hybridizes to a nucleic acid molecule containing a single nucleotide polymorphism (SNP) in any one of the nucleotide sequences in SEQ ID NOS:
- 1-67 and 135-504, wherein the SNP is as specified in Table 1 and Table 2, respectively.
A method of detecting a variant polypeptide, comprising contacting a reagent with a variant polypeptide encoded by a nucleotide sequence containing a single nucleotide polymorphism (SNP) in any one of the nucleotide sequences of SEQ ID NOS:
- 1-67 and 135-504 in a test sample, wherein the SNP is as specified in Table 1 and Table 2, respectively, and detecting the binding of the reagent to the polypeptide.
A method for identifying an individual who is in need of receiving treatment for MI, comprising detecting a single nucleotide polymorphism (SNP) in a sample from said individual in any one of the nucleic acid sequences of SEQ ID NOS:
- 1-67 and 135-504, wherein the SNP is as specified in Table 1 and Table 2, respectively, and treating that individual with a therapeutic agent.
Dependencies are inferred from the claim text.