Microarray and methods of using same
OwnerMei Xuinventor
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Abstract
A DNA microarray device having probes immobilized to the microarray surface, in which targets to be detected using the microarray are exposed to the probes to hybridize therewith. There are provided on the microarray a quantity of a group of perfect match probes for a target of interest such that the molar ratio of each such probe to the target fragment is at least about 20:1.
Claims15
An improved DNA microarray device comprising probes immobilized to the microarray surface, in which targets to be detected using the microarray are exposed to the probes to hybridize therewith, the improvement comprising providing on the microarray a quantity of a group of perfect match probes for a target of interest such that the molar ratio of each probe to the target fragment is at least about 20:1.
A method of determining the presence of a target sequence in a sample that is exposed to a microarray having perfect match probes coupled to its surface, comprising hybridizing under the same conditions a control sample and a test sample, with a series of perfect match probes available for the target sequence, and then comparing the measurements from both samples.
A method of determining the concentration of a target sequence in a sample that is exposed to a microarray having perfect match probes immobilized to its surface, comprising testing the sample and at least two different dilutions thereof under the same hybridization conditions, and comparing the data to determine the target concentration in the sample.
Dependencies are inferred from the claim text.