US 2006/0223086 A1 · Free reactant use in nucleic acid-templated synthesis

Abstract

The present invention provides methods and compositions for expanding the scope of chemical reactions that can be performed during nucleic acid-templated organic syntheses. In particular, nucleic acid-templated chemistries are used to produce reaction intermediates attached to an oligonucleotide that can be used to identify the reaction intermediates and/or the resulting reaction products. The reaction intermediates then are reacted with free reactants (for example, reactants that are difficult or impractical to couple to an oligonucleotide) to produce a reaction product. This approach expands the scope of reagents useful in nucleic acid-templated syntheses to reagents that do not need to be or cannot be tethered to an oligonucleotide. The reagents, however, still permit the synthesis of reaction products attached to oligonucleotides that can be used to identify the reaction products.

Claims23

Dependent claims

A method of synthesizing a reaction product, the method comprising the steps of:

(a) providing a mixture comprising a first reactive unit and a second reactive unit under conditions to induce a reaction between the first and second reactive units to produce a reaction intermediate;
(b) providing an oligonucleotide comprising an identifying sequence covalently attached to the reaction intermediate;
and
(c) combining the reaction intermediate with a free reactant selectively reactive with the reaction intermediate, thereby synthesizing a reaction product linked to the identifying sequence, wherein the free reactant is more reactive with the reaction intermediate than with at least one of the reactive units in the starting mixture.

1 more element

A method of synthesizing a reaction product by nucleic acid-templated synthesis, the method comprising the steps of:

(a) providing a mixture comprising a plurality of first reactive units attached to first oligonucleotides comprising codon sequences, wherein the oligonucleotide sequence is indicative of the first reactive unit attached thereto;
(b) providing a second reactive unit attached to a second oligonucleotide comprising an anti-codon sequence complementary to the codon sequence of at least one first reactive unit;
(c) annealing the codon sequence of at least one of the first oligonucleotides with the anti-codon sequence of the second oligonucleotide to induce a reaction between the first and second reactive units to form a first reaction intermediate attached at least to a first oligonucleotide;
and
(d) combining the first reaction intermediate with a free reactant selectively reactive with the first reaction intermediate, thereby synthesizing a first reaction product attached to the first oligonucleotide, wherein the free reactant is more reactive with the first reaction intermediate than with at least one of the reactive units in the mixture.

2 more elements

Dependencies are inferred from the claim text.

Free reactant use in nucleic acid-templated synthesis

Life of this patent

Free reactant use in nucleic acid-templated synthesis

Life of this patent

Abstract

Claims23