Methods and compositions for detecting Bacillus anthracis
AssigneeBioVeris Corporation
InventorDavid Carlson
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods, compositions, and kits for detecting the presence of B. anthracis and binding partners to B. anthracis. The invention also relates to polynucleotide sequences that are specific for the B. anthracis genome and proteins encoded by those sequences.
Claims111
A method for detecting Bacillus anthracis comprising:
- (a) providing a sample suspected of containing B. anthracis;
- (b) forming a composition comprising nucleic acid from the sample, at least one first primer, and at least one second primer;
- (c) amplifying any nucleic acid which the primers in step (b) can amplify;
- and
- (d) detecting B. anthracis by detecting the amplification products of step (c),
- (i) wherein at least one primer comprises a nucleotide fragment that is substantially identical to a portion of any of SEQ ID NO. 1, 2, 3, or their complements and wherein the at least one primer specifically binds to B. anthracis DNA or RNA and not to any of B. cereus, B. thuringiensis, and B. subtilis DNA or RNA;
- and/or (ii) wherein at least one primer comprises at least 12 contiguous nucleotides that are substantially identical to a portion of SEQ ID NO. 1, 2, 3, or their complements.
A method for detecting B. anthracis comprising:
- (a) providing a sample suspected of containing B. anthracis;
- (b) contacting nucleic acid from the sample with at least one probe under conditions favorable for hybridization;
- and
- (c) detecting B. anthracis in the sample based on the hybridization products of step (b);
- (i) wherein at least one probe comprises a nucleotide fragment that is substantially identical to a portion of any of SEQ ID NO. 1, 2, 3, or their complements and wherein the at least one probe specifically binds to B. anthracis DNA or RNA and not to any of B. cereus, B. thuringiensis, and B. subtilis DNA or RNA and/or (ii) wherein at least one probe comprises at least 12 contiguous nucleotides that are substantially identical to a portion of SEQ ID NO. 1, 2, 3, or their complements.
An isolated polynucleotide comprising a section that is (i) substantially identical to a portion of the nucleotide sequence of any of SEQ ID NOS. 1, 2, 3, or their complements, and wherein the polynucleotide specifically binds to B. anthracis DNA or RNA and not to any of B. cereus, B. thuringiensis, and B. subtilis DNA or RNA and/or (ii) substantially identical to at least 12 contiguous nucleotides of any of SEQ ID NOS. 1, 2, 3, or their complements.
A pair of oligonucleotide primers for use in the amplification-based detection of B. anthracis, wherein each primer comprises at least 12 contiguous nucleotides that are substantially identical to a portion of any of the following six sequences:
- SEQ ID NO. 1, 2, 3, and their complements.
An oligonucleotide probe for use in hybridization-based detection of B. anthracis, wherein the probe comprises at least 12 contiguous nucleotides that are substantially identical to a portion of any of the following six sequences:
- SEQ ID NO. 1, 2, 3, and their complements, and wherein the probe specifically binds to B. anthracis DNA or RNA and not to any of B. cereus, B. thuringiensis, and B. subtilis DNA or RNA.
A kit for the detection of B. anthracis, the kit comprising at least one pair of oligonucleotide primers, wherein each primer comprises at least 12 contiguous nucleotides that are substantially identical to a portion of any of the following six sequences:
- SEQ ID NO. 1, 2, 3, and their complements, and wherein at least one primer specifically binds to B. anthracis DNA or RNA and not to any of B. cereus, B. thuringiensis, and B. subtilis DNA or RNA.
A kit for the detection of B. anthracis, the kit comprising at least one oligonucleotide probe, wherein the probe comprises at least 12 contiguous nucleotides that are substantially identical to a portion of the following six sequences:
- SEQ ID NO. 1, 2, 3, and their complements, and wherein the probe specifically binds to B. anthracis DNA or RNA and not to any of B. cereus, B. thuringiensis, and B. subtilis DNA or RNA.
99-106. (canceled)
A method for amplifying Bacillus anthracis nucleic acid comprising:
- (a) providing a sample of B. anthracis nucleic acid;
- (b) forming a composition comprising the nucleic acid and a primer pair;
- (c) amplifying the nucleotide sequence between the first primer of the primer pair and the second primer of the primer pair to form an amplicon;
- and
- (d) optionally detecting the amplicon,
- (i) wherein at least one primer of the primer pair comprises a nucleotide fragment that is substantially identical to a portion of any of SEQ ID NO. 1, 2, 3, or their complements and wherein the at least one primer specifically binds to B. anthracis DNA or RNA and not to any of B. cereus, B. thuringiensis, and B. subtilis DNA or RNA and/or (ii) wherein at least one primer of the primer pair comprises at least 12 contiguous nucleotides that are substantially identical to a portion of SEQ ID NO. 1, 2, 3, or their complements.
118-126. (canceled)
Dependencies are inferred from the claim text.