Back to results
US 2006/0223080 A1Published application
Compositions and methods for detecting group a streptococci
AssigneeGen-Probe Incorporated
InventorsReinhold Pollner, Paul Darby
Life of this patent
Estimating the term…
Abstract
Compositions, methods and kits for detecting Group A streptococci. Particularly described are oligonucleotides that are useful as amplification primers and hybridization probes for detecting very low levels of Group A streptococci nucleic acids.
Claims16
A hybridization assay probe for detecting a Streptococcus pyogenes nucleic acid comprising:
- a probe sequence that comprises
- a target-complementary sequence of bases, and optionally one or more base sequences that are not complementary to said nucleic acid that is to be detected,
- wherein said target-complementary sequence of bases consists of 13-22 contiguous bases contained within the sequence of SEQ ID NO:3, or the complement thereof, allowing for the presence of RNA and DNA equivalents and nucleotide analogs, and
- wherein said hybridization assay probe has a length of up to 30 bases and does not comprise any other base sequences that hybridize to nucleic acid derived from Streptococcus pyogenes.
1 more element
A kit for amplifying a Streptococcus pyogenes nucleic acid sequence that may be present in a biological sample comprising:
- a first primer that comprises a 3′ terminal target-complementary sequence and optionally a first primer upstream sequence that is not complementary to said Streptococcus pyogenes nucleic acid sequence that is to be amplified, said 3′ terminal target-complementary sequence of said first primer comprising 20 contiguous bases contained within SEQ ID NO:2, allowing for the presence of RNA and DNA equivalents and nucleotide analogs;
- and
- a second primer that comprises a 3′ terminal target-complementary sequence and optionally a second primer upstream sequence that is not complementary to said Streptococcus pyogenes nucleic acid sequence that is to be amplified, said 3′ terminal target-complementary sequence of said second primer comprising 26 contiguous bases contained within SEQ ID NO:1, allowing for the presence of RNA and DNA equivalents and nucleotide analogs.
Dependencies are inferred from the claim text.