Back to results
US 2006/0223079 A1Published application
Electrocatalytic nucleic acid hybridization detection
AssigneeThe Trustees of Boston College
InventorsShana Kelley, Melissa Lapierre-Devlin, Meaghan O'Keefe
Life of this patent
Estimating the term…
Abstract
The detection of specific DNA sequences using electrochemical readout would permit the rapid and inexpensive detection and identification of bacterial pathogens and the analysis of human genes. A new assay developed for this purpose is described that harnesses an electrocatalytic process to monitor DNA hybridization.
Claims20
An electrocatalytic hybridization assay comprising:
- providing a nucleic acid probe engaged to a conductive solid support;
- contacting the nucleic acid probe with a sample wherein the sample comprises a target nucleic acid, a first transition metal complex and a second transition metal complex;
- and
- measuring an electrocatalystic signal generated by hybridization of the nucleic acid probe and the nucleic acid target,
- wherein the presence of the second transition metal complex provides an enhanced electrocatalytic signal.
2 more elements
A method of detecting hybridization between a nucleic acid probe and a target nucleic acid, comprising:
- providing a nucleic acid probe engaged to a conductive solid support;
- contacting the nucleic acid probe with a sample wherein the sample comprises a target nucleic acid, a first transition metal complex and a second transition metal complex;
- and
- measuring an electrocatalystic signal generated by hybridization of the nucleic acid probe and the nucleic acid target.
1 more element
A method of detecting hybridization between a nucleic acid probe and a target nucleic acid, comprising:
- providing a nucleic acid probe engaged to a conductive solid support;
- contacting the nucleic acid probe with a sample wherein the sample comprises a target nucleic acid, a first transition metal complex and a second transition metal complex wherein the first transition metal complex is a Ru(NH3)6−3 complex and the second transition metal complex is a Fe(CN)6−3 complex;
- and
- measuring an electrocatalystic signal generated by hybridization of the nucleic acid probe and the nucleic acid target.
1 more element
Dependencies are inferred from the claim text.