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US 2006/0223071 A1Published application
Methods, compositions, and kits for detecting nucleic acids in a single vessel
OwnerMichele Wisniewski, William Barbour +1inventors
InventorsMichele Wisniewski, William Barbour, Brendan Boyd
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Abstract
The invention encompasses processes, compositions, and kits for isolating and detecting nucleic acids from samples using a metal oxide coated onto a vessel. The nucleic acids can be processed and detected within this vessel.
Claims62
A method for the detection of a microorganism comprising:
- (a) lysing a microorganism to release a nucleic acid molecule;
- (b) contacting the nucleic acid molecule with a vessel coated with at least one metal oxide;
- (c) allowing the nucleic acid molecule to attach to the metal oxide;
- (d) amplifying, in the vessel, a fragment of the nucleic acid molecule attached to the metal oxide to generate an amplified product;
- and
- (e) detecting, in the vessel, the amplified product.
3 more elements
A kit for detecting nucleic acids in biological sample comprising:
- (a) a vessel coated with at least one metal oxide;
- (b) instructions for binding a nucleic acid molecule to the metal oxide;
- and
- (c) instructions for amplifying and detecting, in the vessel, a nucleic acid molecule bound to the metal oxide.
1 more element
A method for the detection of a nucleic acid comprising:
- (a) attaching a nucleic acid molecule to a metal oxide coated onto a vessel;
- (b) amplifying, in the vessel, a fragment of the nucleic acid molecule to generate an amplified product;
- and
- (c) detecting, in the vessel, the amplified product.
1 more element
Dependencies are inferred from the claim text.