Mutant DNA polymerases and methods of use
OwnerPaolo Vatta, John Brandis +2inventors
InventorsPaolo Vatta, John Brandis, Elena Bolchakova, Sandra Spurgeon
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Abstract
The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention.
Claims31
A mutant DNA polymerase comprising an Asn residue at amino acid 543 and a 5′-3′ exonuclease activity reducing mutation, wherein the positions of amino acids of the mutant DNA polymerase are defined with respect to Taq DNA polymerase.
Dependencies are inferred from the claim text.