Methods of nucleic acid analysis
OwnerKnut Rudiinventor
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Abstract
The present invention relates to a method of characterising a target base in a sample nucleic acid, which method comprises: (a) contacting the sample nucleic acid with an oligonucleotide primer under conditions which allow hybridisation of the oligonucleotide to the sample nucleic acid, said oligonucleotide primer being labelled with a fluorophore; (b) contacting the sample nucleic acid with a deoxynucleotide or dideoxynucleotide which is labelled with a fluorophore, under conditions which allow extension of the oligonucleotide primer through incorporation of the labelled nucleotide; and (c) measuring the fluorescence emitted by one or both of the fluorophores.
Claims21
A method of characterising a target base in a sample nucleic acid, which method comprises:
- (a) contacting the sample nucleic acid with an oligonucleotide primer under conditions which allow hybridisation of the oligonucleotide to the sample nucleic acid, said oligonucleotide primer being labelled with a fluorophore;
- (b) contacting the sample nucleic acid with a deoxynucleotide or dideoxynucleotide which is labelled with a fluorophore, under conditions which allow extension of the oligonucleotide primer through incorporation of the labelled nucleotide;
- and
- (c) measuring the fluorescence emitted by one or both of the fluorophores.
Dependencies are inferred from the claim text.