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OwnerKevin Flanigan, Robert Weiss +2inventors
InventorsKevin Flanigan, Robert Weiss, Diane Dunn, Andrew Niederhausern
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Disclosed is a Single Condition Amplification/Internal Primer (SCAIP) sequencing method which allows for the rapid, accurate, and economical analysis of any large multi-exon gene. The method can be used to detect genomic mutations in any large multi-exon gene including the dystrophin gene. In some forms, the method can rely on amplification of a large number of exons at a single set of PCR temperatures with a first set of amplification primers followed by sequencing without optimization of individual amplicon conditions, using a second, internal set of sequencing primers. The SCAIP method provides for the identification and analysis of specific individual genomic mutations such as deletions, point mutations, frameshifts, or combinations thereof, in gene complexes with multiple exons/introns spanning large genomic regions.
A method for characterizing a nucleic acid region, the method comprising
A primer set which recognizes a single exon or a proximal promoter for the dystrophin gene, the set comprising the primers as shown in Table 1 or Table 6.
A primer set which recognizes a single exon or a proximal promoter for the dystrophin gene, the set comprising the primers as shown in Table 2 or Table 7.
Dependencies are inferred from the claim text.