Method for detecting analytes
AssigneeCibitest Gmbh & Co. KG
InventorsJohannes Bonenberger, Sigrid Hagenmaier, Jitka Polackova, Jan Kampen
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Abstract
The present invention relates to a method and devices for detecting analytes comprising the following stages: incubation of a sample with macromolecules, to each of which at least 2 molecules of the analyte to be detected in the sample are coupled; subsequent incubation of the sample with a solid carrier, to which capture molecules for the analyte to be detected are coupled; addition of a fluorescence dye to stain the macromolecules and detection of analytes present in the sample by excitation of the fluorescence dye.
Claims17
A method for detecting analytes comprising the following steps of:
- a) incubating a sample with macromolecules, to each of which at least 2 molecules of the analyte to be detected in the sample are coupled;
- b) further incubating the sample with a solid carrier, to which capture molecules for the analyte to be detected are coupled;
- c) adding a fluorescence dye to stain the macromolecules;
- and
- d) detecting the analytes present in the sample by excitation of the fluorescence dye.
A method for detecting analytes comprising the steps:
- a) incubating a sample with fluorescence-dye-marked macromolecules, to each of which at least 2 molecules of the analyte to be detected in the sample are coupled;
- b) further incubating the sample with a solid carrier, to which capture molecules for the analyte to be detected are coupled;
- and
- c) detecting analytes present in the sample by excitation of the fluorescence dye.
A device comprising a light source fitted on one side of a solid carrier inserted into the device, that a filter disposed respectively between the light source and the solid carrier on the other side of the solid carrier, wherein the device is designed in such a manner that light passing through the solid carrier passes through an aperture into the human eye or into an optical instrument.
Dependencies are inferred from the claim text.