Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of HIV-1

A pair of oligonucleotide primers, for use as a primer set in the amplification of a target sequence located within the LTR region of the genome of HIV-1, said pair consisting of a first oligonucleotide being 10-50 nucleotides in length and comprising 10 sequential nucleotides of a nucleotide sequence selected from the group consisting of:

• SEQ ID NO:1:
• G GGC GCC ACT GCT AGA GA;
• SEQ ID NO:2:
• G TTC GGG CGC CAC TGC TAG A;
• and
• SEQ ID NO:3:
• CGG GCG CCA CTG CTA,
• and a second oligonucleotide being 10-50 nucleotides in length and comprising 10 sequential nucleotides of a nucleotide sequence selected from the group consisting of:
• SEQ ID NO:4:
• CTG CTT AAA GCC TCA ATA AA;
• SEQ ID NO:5:
• CTC AAT AAA GCT TGC CTT GA;
• and
• SEQ ID NO:12:
• GAT GCA TGC TCA ATA AAG CTT GCC TTG
• AGT.

A pair of oligonucleotide primers, for use as a primer set in the amplification of a target sequence located within the LTR region of the genome of HIV-1, said pair consisting of a first oligonucleotide, which hybridizes to the target sequence or its complement, being 10-26 nucleotides in length and comprising 10 sequential nucleotides of a nucleotide sequence selected from the group consisting of:

• SEQ ID NO:1:
• G GGC GCC ACT GCT AGA GA;
• SEQ ID NO:2:
• G TTC GGG CGC CAC TGC TAG A;
• and
• SEQ ID NO:3:
• CGG GCG CCA CTG CTA,
• and a second oligonucleotide, which hybridizes to the target sequence or its complement, being 10-26 nucleotides in length and comprising 10 sequential nucleotides of a nucleotide sequence selected from the group consisting of:
• SEQ ID NO:4:
• CTG CTT AAA GCC TCA ATA AA;
• SEQ ID NO:5:
• CTC AAT AAA GCT TGC CTT GA;
• and
• SEQ ID NO:12:
• GAT GCA TGC TCA ATA AAG CTT GCC TTG
• AGT.