Methods and compositions for treatment of viral lnfection
OwnerPeter Howley, Jianxin Youinventors
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Abstract
The present invention relates to methods and compositions for the treatment and/or prevention of diseases or disorders associated with viral infection. Accordingly, the nucleotide and amino acid sequences for a human Brd4 protein are provided. Also provided are complexes comprising Brd4 and an E2 protein or a functional equivalent of an E2 protein.
Claims14
A polypeptide consisting essentially of an amino acid sequence that is at least about 90% identical to amino acids 1047-1362, 1134-1362 or 1224-1362 of SEQ ID NO:
- 2, wherein the polypeptide binds to E2 or to a latency-associated nuclear antigen (LANA) protein from a Kaposi sarcoma-associated herpesvirus (KSHV).
A complex or composition comprising a polypeptide comprising an amino acid sequence that is at least about 90% identical to SEQ ID NO:
- 2 or 4 or a portion thereof sufficient for binding to an E2 protein or to a LANA protein;
- and an E2 protein or LANA protein or a portion thereof sufficient for binding to a polypeptide comprising SEQ ID NO:
- 2 or 4.
A method for identifying an agent that disrupt the binding of a Brd4 protein to an E2 protein or LANA protein, comprising (i) contacting a protein comprising an amino acid sequence that is at least about 90% identical to SEQ ID NO:
- 2 or 4 or a portion thereof sufficient for binding to an E2 protein and an E2 or LANA protein or a portion thereof that is sufficient for binding to a Brd4 protein in the presence of a test agent;
- and (ii) determining the level of interaction between the two proteins or portion thereof, wherein a lower level of interaction in the presence of the test agent relative to its absence indicates that the test agent is an agent that disrupts the interaction between a Brd4 protein and an E2 protein.
Dependencies are inferred from the claim text.