Methods and compositions for selecting cells with increased potency
AssigneeStratatech Corporation
InventorsJohn Centanni, Lynn Allen-Hoffmann
Life of this patent
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Abstract
The present invention relates generally to compositions for wound closure. More specifically, the present invention provides human skin equivalents engineered to express exogenous polypeptides (e.g., antimicrobial polypeptides and keratinocyte growth factor 2) and compositions and methods for making human skin equivalents engineered to express exogenous polypeptides. In addition, the present invention provides methods for treatment of wounds with human skin equivalents engineered to express exogenous polypeptides.
Claims14
1-80. (canceled)
A method of selecting cells with increased pluripotency or multipotency relative to a population, comprising;
- a) providing a population of cells;
- b) electroporating said cells under conditions such that electroporated cells with increased pluripotency or multipotency relative to said population of cells are selected.
A method of selecting keratinocytes with holoclone or meroclone cell morphology, comprising;
- a) providing a population of keratinocytes;
- b) electroporating said keratinocytes under conditions such that electroporated keratinocytes with holoclone or meroclone cell morphology are selected.
Dependencies are inferred from the claim text.