Internal ribosome entry site of the labial gene for protein expression
AssigneeNational Health Research Institutes
InventorsDung-Fang Lee, Jyh-Lyh Juang
Life of this patent
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Abstract
The invention describes compositions and methods for recombinant protein expression in a wide range of cell types. The compositions comprise an IRES sequence from the Drosophila labial (lab) gene, or a variant or fragment thereof, or alternatively, a homolog of a lab IRES, or a variant or fragment thereof. Methods of using the compositions are also described.
Claims27
-5. (canceled)
A nucleic acid vector for the expression of at least two cistrons comprising:
- a. a promoter operably linked to a nucleotide sequence comprising at least two cistrons;
- and
- b. at least one nucleotide sequence comprising a homolog of SEQ ID NO. 1, or a variant or fragment thereof, operably linked to at least one of said at least two cistrons, wherein said homolog of SEQ ID NO. 1, or variant or fragment thereof, provides IRES activity.
-14. (canceled)
(canceled)
-26. (canceled)
A baculovirus transfer vector for the expression of at least two cistrons comprising:
- a. a polyhedrin promoter operably linked to a nucleotide sequence comprising at least two cistrons;
- and
- b. at least one nucleotide sequence comprising a homolog of SEQ ID NO. 1, or a variant or fragment thereof, operably linked to at least one of said at least two cistrons, wherein said homolog, or variant or fragment thereof provides IRES activity.
A recombinant baculovirus capable of expressing at least two cistrons in a host cell comprising a baculovirus genome comprising:
- a. a polyhedrin promoter operably linked to a nucleotide sequence comprising at least two cistrons;
- and
- b. at least one nucleotide sequence comprising a homolog of SEQ ID NO. 1, or a variant or fragment thereof operably linked to at least one of said at least two cistrons, wherein said homolog, or variant or fragment thereof, provides IRES activity.
Dependencies are inferred from the claim text.