Compositions and methods for delivery of genetic material

A method of inducing antibodies against an antigen in an individual comprising the steps of:

• injecting into tissue of said individual at a site on said individual's body, a DNA molecule and a polynucleotide function enhancer,
• said DNA molecule comprising a DNA sequence that encodes an antigen, said DNA sequence operatively linked to regulatory sequences which control the expression of said DNA sequence,
• said polynucleotide function enhancer is a compound having one of the following formula:
• Ar—R1—O—R2—R3
• or
• Ar—N—R1—R2—R3
• or
• R4—N—R5—R6
• or
• R4—O—R1—R7
• wherein:
• Ar is benzene, p-aminobenzene, m-aminobenzene, o-aminobenzene, substituted benzene, substituted p-aminobenzene, substituted m-aminobenzene, substituted o-aminobenzene, wherein the amino group in the aminobenzene compounds can be amino, C1-C5 alkylamine, C1-C5, C1-C5 dialkylamine and substitutions in substituted compounds are halogen, C1-C5 alkyl and C1-C5 alkoxy;
• R1 is C═O;
• R2 is C1-C10 alkyl including branched alkyls;
• R3 is hydrogen, amine, C1-C5 alkylamine, C1-C5, C1-C5 dialkylamine;
• R2+R3 can form a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle including a C1 -C10 alkyl N-substituted heterocycle;
• R4 is Ar, R2 or C1-C5 alkoxy,a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle and a C1-C10 alkoxy substituted heterocycle including a C1-C10 alkyl N-substituted heterocycle;
• R5 is C═NH;
• R6 is Ar, R2 or C1-C5 alkoxy, a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle and a C1-C10 alkoxy substituted heterocycle including a C1-C10 alkyl N-substituted heterocycle;
• and,
• R7 is Ar, R2 or C1-C5 alkoxy, a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle and a C1-C10 alkoxy substituted heterocycle including a C1-C10 alkyl N-substituted heterocycle;
• and,
• wherein said DNA molecule is taken up by cells in said tissue, said DNA sequence is expressed in said cells and an antibody is generated against said antigen.