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US 2006/0219638 A1Published application
Method and device for multidimensional liquid chromatography
AssigneeHitachi High-Technologies Corporation
InventorsMasaki Watanabe, Shinya Ito, Katsuhiro Kanda
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Abstract
In a liquid chromatography, an electrolytic solution starts to be supplied from an injector toward a specimen in a first separating column to transfer components of the specimen from the first separating column toward one of first and second trap columns after a time period more than zero elapses from a time at which another eluting liquid starts to flow through the other one of the first and second trap columns toward a second separating column.
Claims18
A liquid chromatography device comprising:
- an injector for injecting a specimen into an eluting liquid,
- a first separating column for holding the specimen before and when an electrolytic solution is supplied to the specimen in the first separating column so that components of the specimen are extracted by the electrolytic solution from the specimen in the first separating column,
- a first pump for feeding the eluting liquid to transfer the specimen from the injector to the first separating column and feeding the electrolytic solution to extract the components of the specimen from the specimen in the first separating column,
- first and second trap columns each of which is capable of holding the components of the specimen extracted from the specimen in the first separating column,
- a second pump for feeding another eluting liquid to one of the first and second trap columns after the components of the specimen are held by the one of the first and second trap columns,
- a second separating column for holding, when at least one of the components of the specimen transferred with the another eluting liquid from the one of the first and second trap columns to the second separating column is eluted by the another eluting liquid from the components thereof in the second separating column, remainder at least one of the components of the specimen, and
- a detector for detecting the one of the components of the specimen eluted from the components thereof in the second separating column.
4 more elements
A method for detecting at least one of components of a specimen by a liquid chromatography device including:
- an injector for injecting the specimen into an eluting liquid,
- a first separating column for holding the specimen before and when an electrolytic solution is supplied to the specimen in the first separating column so that the components of the specimen are extracted by the electrolytic solution from the specimen in the first separating column,
- a first pump for feeding the eluting liquid to transfer the specimen from the injector to the first separating column and feeding the electrolytic solution to extract the components of the specimen from the specimen in the first separating column,
- first and second trap columns each of which is capable of holding the components of the specimen extracted from the specimen in the first separating column,
- a second pump for feeding another eluting liquid to one of the first and second trap columns after the components of the specimen are held by the one of the first and second trap columns,
- a second separating column for holding, when the at least one of the components of the specimen transferred with the another eluting liquid from the one of the first and second trap columns to the second separating column is eluted by the another eluting liquid from the components thereof in the second separating column, remainder at least one of the components of the specimen, and
- a detector for detecting the at least one of the components of the specimen eluted from the components thereof in the second separating column,
- comprising the steps of:
- a first step during which the eluting liquid is allowed to flow from the first pump through the first separating column to the one of the first and second trap columns and the another eluting liquid is allowed to flow from the second pump through the other one of the first and second trap columns to the second separating column, and
- a second step during which the eluting liquid is allowed to flow from the first pump through the one of the first and second trap columns while preventing the eluting liquid from flowing through the first separating column and the another eluting liquid is allowed to flow from the second pump through the other one of the first and second trap columns to the second separating column.
7 more elements
Dependencies are inferred from the claim text.