Siloxane-immobilized particulate stationary phases for chromatographic separations and extractions
AssigneeWaters Investments Limited
InventorsRobert Plumb, Jennifer Granger
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Abstract
Chromatography and solid phase extraction devices having immobilized stationary phases are disclosed. An exemplary device of the Invention includes a column or cartridge packed with a mixture of a particulate stationary phase material and a polymeric network of Cross-linked poly(diorganosiloxane), e.g., poly(dimethylsiloxane). The Invention also provides methods of making and using such devices.
Claims62
An immobilized stationary phase in a chromatography column comprising an intimate mixture of particles comprising a stationary phase material and a polymeric network comprising cross-linked poly(diorganosiloxane), wherein said particles are suspended in said network.
A medium for molecular separations or extractions comprising an intimate mixture of particles comprising a stationary phase material and a polymeric network comprising cross-linked poly(diorganosiloxane), and wherein said particles are suspended in said network.
A chromatography device comprising
- a) a column having a cylindrical interior for accepting a stationary phase;
- and
- b) an immobilized particulate stationary phase packed within said column;
- wherein said immobilized stationary phase comprises an intimate mixture of particles comprising a stationary phase material and a polymeric network comprising cross-linked poly(diorganosiloxane), and wherein said particles are suspended in said network.
49-94. (canceled)
97-99. (canceled)
A method of making a packed HPLC column comprising the steps of
- a) providing a stainless steel column having a cylindrical interior for accepting a stationary phase;
- b) placing a mixture of particulate stationary phase material, a compatible solvent, and polymer reagents into said stainless steel wherein said polymer reagents are capable of forming by cross-linking reactions a polymeric network of poly(diorganosiloxane);
- c) applying high pressure to compress or pack said mixture within said column;
- and
- d) curing said mixture within said column while maintaining high pressure to cross-link said poly(diorganosiloxane) and thereby produce an immobilized stationary phase.
A solid phase extraction apparatus comprising a hollow body having an input port, an output port, and an immobilized a stationary phase located therein, wherein said immobilized stationary phase is an intimate mixture of particles comprising a stationary phase material and a polymeric network comprising cross-linked poly(diorganosiloxane), wherein said particles are suspended in said network.
Dependencies are inferred from the claim text.