Improved Methods and Devices for Concentration and Fractionation of Analytes for Chemical Analysis including Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry (MS)
OwnerDean Hafeman, James Harkins +6inventors
InventorsDean Hafeman, James Harkins, Jeremy Norris, Sheila Baker, Donald Loveday, Daniel Kuban, Richard Caprioli, Charles Witkowski
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Abstract
A device for pre-concentration and purification of analytes from biological samples, such as human serum, to be analyzed by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) and methods of use thereof are provided.
Claims39
A device for electrophoretically separating, concentrating, and capturing an analyte in a sample comprising:
- a sample well for retaining a fluid sample in an electrolyte;
- a separation layer providing a path for diffusive ionic, and fluidic communication with the well;
- and
- a capture layer providing a path for diffusive ionic, and fluidic communication with the separation layer.
A device for capturing a sample analyte for analysis in a mass spectrometer comprising:
- a cartridge capture slide comprising a plurality of apertures disposed therein;
- a plurality of capture layers disposed in the plurality of apertures.
A cartridge capture slide adaptable for use in a mass spectrometer comprising:
- an array of apertures disposed in the cartridge capture slide;
- and
- an array of capture layers disposed in the array of apertures,
- wherein the cartridge capture slide is incorporated within a device comprising an array of sample wells and a cartridge gel plate, and wherein the cartridge gel plate comprises an array of apertures in which are disposed an array of separation layers.
Dependencies are inferred from the claim text.