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US 2006/0218674 A1Published application
Alteration of plant embryo/endosperm size during development
AssigneeE.I. du Pont de Nemours and Company
InventorsHajime Sakai, Nobuhiro Nagasawa
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Abstract
Isolated nucleic acid fragments and recombinant constructs comprising such fragments useful for altering embryo/endosperm size during seed development are disclosed along with a method of controlling embryo/endosperm size during seed development in plants using such recombinant constructs.
Claims19
An isolated polynucleotide comprising:
- (a) a nucleic acid sequence encoding a polypeptide involved in altering embryo/endosperm size during seed development, said polypeptide having at least 80% amino acid sequence identity, based on the Clustal V method of alignment, when compared to an amino acid sequence selected from the group consisting of SEQ ID NOS:37, 39, 41, 43, 45, 47, 49, 51, and 53;
- or
- (b) a nucleic acid sequence set forth in SEQ ID NO:25 wherein said sequence comprises at least one of the following modifications:
- (i) nucleotide 271 is a T residue instead of a C;
- (ii) nucleotide 110 is a T residue instead of a G;
- or
- (iii) nucleotide 75 is deleted;
- or
- (c) a nucleic acid sequence set forth in SEQ ID NO:34 wherein
- (i) nucleotides 4473 through 4829 correspond to a first exon, and
- (ii) nucleotides 5661 through 6110 correspond to a second exon, and
- further wherein the nucleotides of (c) (i) and/or (c)(ii) encode a polypeptide involved in altering embryo/endosperm size during seed development;
- or
- (d) a nucleic acid sequence set forth in SEQ ID NO:72;
- or
- (e) a full complement of (a), (b), (c), (d), or SEQ ID NO:34;
- or
- (f) all or part of a non-coding or coding region of the isolated polynucleotide comprising sequences of (a), (b), (c), (d), (e), or SEQ ID NO:34 for use in co-suppression or antisense suppression of endogenous nucleic acid sequences encoding polypeptides involved in altering embryo/endosperm size during seed development.
15 more elements
A method of mapping genetic variations related to controlling embryo/endosperm size and/or altering oil phenotype in plants comprising:
- (a) crossing two plant varieties;
- and
- (b) evaluating genetic variations with respect to
- (i) a nucleic acid sequence selected from the group consisting of SEQ ID NOS:25, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52 and 72;
- or
- (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOS:26, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 51, and 53;
- in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of RFLP analysis, SNP analysis, and PCR-based analysis.
4 more elements
A method of molecular breeding to control embryo/endosperm size and/or altering oil phenotype in plants comprising:
- (a) crossing two plant varieties;
- and
- (b) evaluating genetic variations with respect to
- (i) a nucleic acid sequence selected from the group consisting of SEQ ID NOS:25, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52 and 72;
- or
- (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOS:26, 29, 31, 33, 30, 32, 34, 36, 38, 40, 42, 44, and 46;
- in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of RFLP analysis, SNP analysis, and PCR-based analysis.
4 more elements
Dependencies are inferred from the claim text.