Back to results
US 2006/0218669 A1Published application
Sialylation of glycoproteins in plants
OwnerLokesh Joshi, Miti Shahinventors
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
OwnerLokesh Joshi, Miti Shahinventors
Estimating the term…
The presence of sialic acids (SAs) at the non-reducing terminal of various glycoconjugates plays important roles in the function and half-life of glycoproteins in mammals and many species of microorganisms. It has been previously unknown that plant cells have the capacity to sialylate glycoproteins. The present invention discloses the presence of sialylated glycoconjugates and identifies N-acetylneuranminic acid (Neu5Ac) and N-glycolylneuraminic (Neu5Gc) on glycoproteins in suspension-cultured cells of plants, for example, Arabidopsis thaliana (A. thaliana), Nicotiana tabacum (tobacco), and Medicago sativa (alfalfa), extending the complexity of glycan structures known in the plant kingdom. This invention further discloses methods for engineering plants to produce recombinant glcyoproteins.
1-47. (canceled)
A method for producing recombinant sialylated glycoproteins in plants, comprising administering a vector comprising a nucleic acid encoding the glycoprotein to a plant cell, wherein the plant cell expresses a plant CMP-sialic acid transporter and a plant sialyltransferase.
A method for engineering plants to produce recombinant sialylated glycoproteins, comprising administering to the plant cell a vector comprising a nucleic acid encoding plant CMP-sialic acid transporter and plant sialyltransferase.
Dependencies are inferred from the claim text.