Nucleotide sequences encoding anthonomous toxic polypeptides from bacillus thuringiensis

An isolated and purified polynucleotide sequence encoding the polypeptide of SEQ ID NO:8.

-18. (canceled)

A vector for use in transforming a host cell, wherein said vector comprises a polynucleotide sequence encoding the polypeptide as set forth in SEQ ID NO:8.

A plant tissue transformed with a polynucleotide sequence which expresses the polypeptide of claim 1, wherein said tissue is selected from the group consisting of a plant cell, an embryonic plant tissue, plant calli, a leaf, a plant stem, a plant root, a plant flower, a fruit, a fruiting body, a boll, and a plant seed.

A method of making a transgenic plant resistant to coleopteran insect infestation comprising incorporating into a genome of a plant cell a polynucleotide comprising at least a plant functional promoter operably linked to a nucleotide sequence encoding the polypeptide of SEQ ID NO:8, isolating and propagating a plant cell transformed with said polynucleotide, regenerating a plant from said plant cell transformed with said polynucleotide, and propagating said plant from progeny, wherein said plant expresses an insecticidally effective amount of said polypeptide from said polynucleotide.

-32. (canceled)

A plant cell transformed with a polynucleotide sequence that expresses one or more of the polypeptides as set forth in SEQ ID NO:2, SEQ ID NO:8, and SEQ ID NO:10 or insecticidal fragments thereof, wherein said cell produces an amount of said one or more polypeptides effective for controlling a Coleopteran insect pest infestation.