Cis-acting regulatory elements from tripsacum dactyloides
AssigneeAthenix Corporation
InventorPhilip Hammer
Life of this patent
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Abstract
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel promoter nucleotide sequence for the gene encoding ubiquitin in Tripsacum dactyloides, as well as vectors, microorganisms, plants and plant cells comprising the promoter nucleotide sequence, or variants and fragments thereof. Methods for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein are also provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Claims23
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6;
- (b) the nucleotide sequence of the DNA insert of the plasmid deposited as Accession No. NRRL B-30819;
- (c) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6, wherein said sequence initiates transcription in a plant cell;
- (d) a nucleotide sequence having at least 80% sequence identity to the sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6, wherein said sequence initiates transcription in a plant cell;
- and,
- (e) a nucleotide sequence that hybridizes under stringent conditions to a) or b), wherein said sequence initiates transcription in a plant cell.
A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant cell an expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6;
- (b) a nucleotide sequence comprising the plant promoter sequence of the DNA insert of the plasmid deposited as Accession No. NRRL B-30819;
- (c) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6, wherein said nucleotide sequence initiates transcription in said plant cell;
- (d) a nucleotide sequence having at least 80% sequence identity to the sequence set forth in SEQ ID NO:1, 2, 3, 5, or 6, wherein said nucleotide sequence initiates transcription in said plant cell;
- and,
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of a) or b), wherein said sequence initiates transcription in said plant cell;
- and, regenerating a transformed plant from said plant cell, wherein said plant has stably incorporated into its genome said expression cassette.
A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell an expression cassette comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6;
- (b) a nucleotide sequence comprising the plant promoter sequence of the DNA insert of the plasmid deposited as Accession No. NRRL B-30819;
- (c) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6, wherein said nucleotide sequence initiates transcription in said plant cell;
- (d) a nucleotide sequence having at least 80% sequence identity to the sequence set forth in SEQ ID NO:1, 2, 3, 5 or 6, wherein said nucleotide sequence initiates transcription in said plant cell;
- and,
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of a) or b), wherein said sequence initiates transcription in said plant cell.
Dependencies are inferred from the claim text.