Preparation of organisms with faster growth and/or higher yield
AssigneeMetanomics GmbH
InventorsGunnar Plesch, Agnes Chardonnens, Oliver Schmitz, Piotr Puzio, Marcus Ebneth, Xi-Qing Wang
Life of this patent
Estimating the term…
Abstract
A method for preparing a nonhuman organism with faster growth and/or increased yield in comparison with a reference organism, which method comprises increasing the activity of L450 in said organism or in one or parts thereof in comparison with a reference organism.
Claims29
A method for preparing a nonhuman organism with faster growth and/or increased yield in comparison with a reference organism, which method comprises increasing the activity of L450 in said organism or in one or more parts thereof in comparison with a reference organism.
A polynucleotide encoding an L450 polypeptide, which comprises a nucleic acid molecule selected from the group consisting of:
- (a) nucleic acid molecule encoding at least the mature form of the polypeptide as depicted in SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 25 27, 35, 37, 39, 41, 43, 45, 46, 47, 48, 49, 50, 51 or 53 or comprising at least the mature form of the polynucleotide depicted in SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 24 26, 34, 36, 38, 40, 42, 44 or 52;
- (b) nucleic acid molecule whose sequence is derivable from a polypeptide sequence encoded by a nucleic acid molecule according to (a) due to the degeneracy of the genetic code;
- (c) nucleic acid molecule encoding an L450 polypeptide whose sequence is at least 30% identical to the amino acid sequence of the polypeptide encoded by the sequence depicted in SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 25 27, 35, 37, 39, 41, 43, 45, 46, 47, 48, 49, 50, 51 or 53 or comprising the sequence depicted in SE ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 24 26, 34, 36, 38, 40, 42, 44 or 52;
- (d) nucleic acid molecule encoding a polypeptide that is derived from an L450 polypeptide encoded by a polynucleotide according to (a) to (c) by substitution, deletion and/or addition of one or more amino acids of the amino acid sequence of the polypeptide encoded by the nucleic acid molecules (a) to (c);
- (e) nucleic acid molecule encoding a fragment or an epitope of the L450 polypeptide encoded by any of the nucleic acid molecules according to (a) to (d);
- (f) nucleic acid molecule comprising a polynucleotide which comprises the sequence of a nucleic acid molecule obtained by amplification of a cDNA bank using the primers in SEQ ID NOs:
- 19 and 20 and/or 28 and 30 or a combination thereof or of a genomic bank using the primers in SEQ ID NOs:
- 21 and 22;
- (g) nucleic acid molecule encoding an L450 polypeptide which has been isolated with the aid of monoclonal antibodies against a polypeptide encoded by any of the nucleic acid molecules according to (a) to (f);
- (h) nucleic acid molecule which is obtainable by screening an appropriate library under stringent conditions using a probe comprising any of the sequences according to (a) to (g) and which encodes an L450 polypeptide,
- (i) nucleic acid molecule encoding a L450 polypeptide comprising the sequence shown in SEQ ID NO:
- 46 or 49, whereby 20 or less of the amino acid positions indicated can be replaced by an X and/or whereby 20 or less amino acids are inserted into the shown sequence or shown in SEQ ID NO:
- 47, 48, 50 or 51, whereby 10 or less of the amino acid positions indicated can be replaced by an X and/or whereby 10 or less amino acids are inserted into the shown sequence;
- or the complementary strand thereof, said polynucleotide or said nucleic acid molecule according to (a) to (h) not comprising the sequence depicted in SEQ ID NO:
- 1, 3, 9, 11, 13, 15, 23, 42 or 44 or the sequence complementary thereto.
A transgenic microorganism having an increased amount of L450.
(canceled)
A method for preparing fine chemicals, which comprises providing a cell, a tissue or a nonhuman organism having increased L450 activity and culturing said cell, said tissue or said organism under conditions which allow production of the desired fine chemicals in said cell, said tissue or said organism.
(canceled)
A method for preparing a nonhuman organism having increased tolerance to stress in comparison with a reference organism, which method comprises increasing the L450 activity in said organism or in one or more parts thereof in comparison with a reference organism.
Dependencies are inferred from the claim text.