Novel selection method
AssigneeBASF Plant Science GmbH
InventorsMichael Kock, Markus Frank, Ralf Badur
Life of this patent
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Abstract
The present invention relates to processes for preparing transformed plant cells or organisms by transforming a population of plant cells which comprises at least one marker protein having a direct or indirect toxic effect for said population, with at least one nucleic acid sequence to be inserted in combination with at least one compound, preferably a DNA construct, capable of reducing the expression, amount, activity and/or function of the marker protein, with the transformed plant cells having a growth advantage over nontransformed cells, due to the action of said compound.
Claims31
A process for preparing transformed plant cells or organisms, comprising:
- a) transforming a population of plant cells, the cells of said population containing at least one marker protein capable of causing directly or indirectly a toxic effect for said population, with at least one nucleic acid sequence inserted in combination with at least one double-stranded marker protein ribonucleic acid sequence or an expression cassette or expression cassettes ensuring expression thereof capable of reducing the expression of at least one marker protein, and
- b) selecting transformed plant cells whose genome contains said nucleic acid sequence and which have a growth advantage over nontransformed cells, due to the action of said double-stranded marker protein ribonucleic acid sequence, from said population of plant cells, the selection being carried out under conditions under which the marker protein can exert its toxic effect on the nontransformed cells.
An amino acid sequence coding for a plant 5-methylthioribose kinase, wherein said amino acid sequence comprises at least one sequence selected from the group consisting of SEQ ID NO:
- 60, 62, 64, 66 and 68.
A nucleic acid sequence coding for a plant 5-methylthioribose kinase, wherein said nucleic acid sequence comprises at least one sequence selected from the group consisting of SEQ ID NO:
- 59, 61, 63, 65 and 67.
A double-stranded RNA molecule, comprising
- a) a “sense” RNA strand comprising at least one ribonucleotide sequence which is essentially identical to at least a part of the “sense” RNA transcript of a nucleic acid sequence coding for a marker protein, and
- b) an “antisense” RNA strand which is essentially complementary to the RNA sense strand under a).
A double-stranded RNA molecule, comprising
- a) a “sense” RNA strand comprising at least one ribonucleotide sequence which is essentially identical to at least a part of the “sense” RNA transcript of a nucleic acid sequence coding for a marker protein, and
- b) an “antisense” RNA strand which is fully complementary to the RNA sense strand under a).
Dependencies are inferred from the claim text.