Method for improving germline transmission efficiency of avian primordial germ cells
AssigneesSeoul National University Industry Foundation, Avicore Biotechnology Institute Inc., Hanmi Pharm. Co., Ltd.
InventorsJae Han, Tae Park, Yeong Hong, Se Kwon
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Abstract
The present invention relates to a method for improving the germline transmission efficiency of avian primordial germ cells (PGCs), and methods for producing avian chimeras and transgenic using it. The present method comprises the steps of (a) isolating primordial germ cells (PGCs) from an avian embryonic gonad; and (b) culturing said PGCs in vitro for at least 5 days. According to the present method, the germline transmission efficiency of PGCs can be dramatically improved in a feasible manner.
Claims17
A method for improving the germline transmission efficiency of avian primordial germ cells (PGCs), which comprises the steps of:
- (a) isolating primordial germ cells (PGCs) from an avian embryonic gonad;
- and (b) culturing said PGCs in vitro for at least 5 days.
A method for preparing an avian germline chimera exhibiting the improved germline transmission efficiency, which comprises the steps of:
- (a) isolating primordial germ cells (PGCs) from an avian embryonic gonad;
- (b) culturing said PGCs in vitro for at least 5 days;
- (c) injecting said cultured PGCs into a recipient embryo;
- and (d) incubating and hatching an egg containing said recipient embryo, whereby the avian germline chimera is prepared.
A method for preparing a transgenic avian exhibiting the improved germline transmission efficiency, which comprises the steps of:
- (a) isolating primordial germ cells (PGCs) from an avian embryonic gonad;
- (b) transferring a foreign gene to said PGCs;
- (c) culturing said transformed PGCs in vitro for at least 5 days;
- (d) injecting said cultured PGCs into a recipient embryo;
- and (e) incubating and hatching an egg containing said recipient embryo, whereby the transgenic avian is prepared.
Dependencies are inferred from the claim text.