Hemipteran muscarinic receptor
OwnerDebra Yuhas, Cancan Huang +4inventors
InventorsDebra Yuhas, Cancan Huang, Susan Gilbey, Valerie Deutsch, Jeffrey Herron, Ruihua Chen
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Abstract
Novel nucleic acid sequences encoding hemipteran muscarinic receptors, and recombinant expressions and host cells comprising the same are disclosed. Isolated hemipteran muscarinic receptors, host cells expressing hemipteran muscarinic receptors, methods of producing the hemipteran muscarinic receptors and antibodies specific for hemipteran muscarinic receptors are also disclosed. Methods of identifying modulators of hemipterana muscarinic receptors are disclosed.
Claims20
A substantially pure protein having the amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 2, a mutant thereof, a fragment thereof, SEQ ID NO:
- 4, a mutant thereof, and a fragment thereof.
An isolated nucleic acid molecule having a nucleic acid sequence selected from the group consisting of:
- SEQ ID NO:
- 1, a fragment thereof having at least 10 nucleotides, SEQ ID NO:
- 3, and a fragment thereof having at least 10 nucleotides.
An isolated nucleic acid molecule consisting of SEQ ID NO:
- 1 or a fragment thereof having at least 10 nucleotides.
An isolated antibody which binds to an epitope on SEQ ID NO:
- 2 or an epitope on SEQ ID NO:
- 4.
A method of identifying a modulator of a hemipteran muscarinic receptor protein activity comprising the steps of:
- performing a test assay by contacting a test cell, which comprises a recombinant expression vector that contains a nucleic acid sequence that encodes said hemipteran muscarinic receptor, wherein said test cell expresses hemipteran muscarinic receptor, with a solution containing calcium in the presence of a test compound, and detecting the amount of intracellular calcium in said test cell;
- performing a negative control assay by contacting a negative control cell which comprises a recombinant expression vector that contains a nucleic acid sequence that encodes said hemipteran muscarinic receptor, wherein said negative control cell expresses hemipteran muscarinic receptor, with a solution containing calcium in the absence of said test compound, and detecting the amount of intracellular calcium in said negative control cell;
- comparing the amount of intracellular calcium in said test cell to the amount of intracellular calcium in said negative control cell
- wherein a change in the amount of and a hemipteran muscarinic receptor agonist calcium in said test cell compared to the amount of intracellular calcium in said negative control cell indicates the test compound is a modulator of a hemipteran muscarinic receptor protein activity.
A method of identifying an inhibitor of a hemipteran muscarinic receptor protein activity comprising the steps of:
- performing a test assay by contacting a test cell, which comprises a recombinant expression vector that contains a nucleic acid sequence that encodes said hemipteran muscarinic receptor, wherein said test cell expresses hemipteran muscarinic receptor, with a solution containing calcium and a hemipteran muscarinic receptor agonist in the presence of a test compound, and detecting the amount of intracellular calcium in said test cell;
- performing a control assay by contacting a negative control cell, which comprises a recombinant expression vector that contains a nucleic acid sequence that encodes said hemipteran muscarinic receptor, wherein said test cell expresses hemipteran muscarinic receptor, with a solution containing calcium and a hemipteran muscarinic receptor agonist in the absence of said test compound, and detecting the amount of intracellular calcium in said negative control cell;
- comparing the amount of intracellular calcium in said test cell to the amount of intracellular calcium in said control cell
- wherein a decrease in the amount of intracellular calcium in said test cell compared to the amount of intracellular calcium in said control cell indicates the test compound is an inhibitor of a hemipteran muscarinic receptor protein activity.
Dependencies are inferred from the claim text.