Method for Producing Insulin From a Natural Source and Insulin Produced by Said Method

A method for producing insulin CHARACTERIZED in that a reindeer pancreas is homogenized in an ethanol hydrochloride solution (1:15), extracted for a two-hour period at room temperature and centrifugation-clarified, a supernatant fluid is subjected to ion-exchange chromatography involving the use of KY-23 microporous sulfocationite in the H+ form, an isoelectric precipitation of the proteins concomitant with the principal matter is performed by successive reduction of the pH value of the eluate resultant from ion-exchange chromatography to 2.0, and further upon adding acetone (20% of a total amount of the solution), to 4.0, whereupon the mixtures are let to stand for an hour at +20° C. and then to stand overnight at +4° C., next a 10-percent zinc-acetate solution (2% of a total amount of the solution) is added to the supernatant fluid to bring the pH value of the solution to 5.95, after which the solution is let to stand at +20° C. for an hour and then put in a cooler for 72 hours, the precipitate is centrifugation-separated to produce insulin which is then purified by high-efficiency reversed-phase liquid co-chromatography.