DNA vaccine formulations

A plasmid DNA formulation which comprises:

• a) purified plasmid DNA whereby metal ions have been optionally removed;
• b) at least one metal ion chelator selected from the group consisting of EDTA, DTPA, NTA, inositol hexaphosphate, tripolyphosphate, polyphosphoric acid, sodium succinate, potassium succinate, lithium succinate, sodium malate, potassium malate, lithium malate and combinations thereof, at a concentration from 0.5 μM to 1000 μM.
• c) ethanol at a weight to volume concentration from 0.5% to 2.5%;
• d) a buffer in the pH range from 7.0 to 9.5;
• wherein the buffer is selected from the group consisting of Tris-HCl, glycine, sodium phosphate, potassium phosphate, lithium phosphate, sodium succinate, potassium succinate, lithium succinate, sodium malate, potassium malate, lithium malate, sodium bicarbonate, potassium bicarbonate, lithium bicarbonate and combinations thereof, at a concentration from about 0.5 mM to about 50 mM;
• and,
• e) a salt selected from the group consisting of NaCl, KCl, LiCl and combinations thereof at a concentration from 50 mM to 300 mM.

A plasmid DNA formulation which comprises:

• (a) purified plasmid DNA whereby metal ions have been optionally removed;
• (b) Tris-HCl buffer at a pH from 7.0 to 9.5;
• (c) ethanol from 0.5% to 2.5% w/v;
• (d) EDTA in a concentration range up to 5 mM;
• and,
• (e) NaCl at a concentration from 50 mM to 300 mM.