Sample preparation method, analysis system and preparation device
AssigneeHitachi High-Technologies Corporation
InventorsKatsuhiro Kanda, Yukie Sasakura, Tsuyoshi Ogino, Makoto Nogami
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Abstract
The object of the present invention is to promote the efficiency and simplify a sample pretreatment step for analysis of molecules contained in the sample. In the present invention, a plurality of various enzymes necessary for pretreatment of sample for analysis of various molecules contained in the sample are immobilized on a solid phase separately for each kind of enzyme, and the enzyme reactions are carried out simultaneously. That is, a sample is reacted with plural kinds of enzymes under the same conditions, and contamination caused by the enzyme reactions is inhibited and the sample is subjected to a given pretreatment.
Claims13
A method for pretreatment of a sample for analysis of the sample which comprises contacting a plurality of molecules to be analyzed which are contained in the sample with an enzyme-immobilized support comprising a solid phase having enzymes immobilized thereon, where the enzyme-immobilized support has two or more kinds of enzymes as a whole.
A system for analysis of a sample in which an enzyme reaction device and a separation and detection device are provided, said enzyme reaction device includes a means for pretreatment and analysis of the sample by contacting a plurality of molecules to be analyzed which are contained in the sample with an enzyme-immobilized support comprising a solid phase having enzymes immobilized thereon, and said enzyme-immobilized support has two or more kinds of enzymes as a whole.
A device for pretreatment of a sample which comprises, as one set, two or more kinds of enzyme-immobilized supports, each of which has a solid phase and enzymes immobilized on the surface of the solid phase.
An enzyme-immobilized support for pretreatment of a sample which comprises one solid phase and two or more kinds of enzymes immobilized separately for each kind of the enzyme on the surface of the solid phase.
Dependencies are inferred from the claim text.