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AssigneesYaeta Endou, Mitsubishi Chemical Corporation
InventorsYaeta Endou, Toshiyuki Kohno
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An object of the present invention is to provide a method for synthesizing, in a cell-free protein synthetic system using a wheat germ extract, a protein of interest that is labeled in an amino-acid-selective manner. The present invention provides a method for carrying out a translation reaction of a protein of interest under a condition whereby a metabolic reaction of alanine to aspartic acid and glutamic acid, a metabolic reaction of aspartic acid to glutamic acid or a metabolic reaction of glutamic acid to aspartic acid and/or glutamine is inhibited, while the protein synthesis is not inhibited, when alanine, aspartic acid or glutamic acid of the protein of interest is labeled with a stable isotope in a cell-free protein synthetic system containing a wheat germ extract.
A method for synthesizing a protein of interest wherein alanine is selectively labeled, which is characterized in that a protein of interest is synthesized in a cell-free protein synthetic system containing a wheat germ extract, under a condition whereby conversion of alanine to aspartic acid and glutamic acid is inhibited while the protein synthesis is not inhibited, by using a substrate wherein alanine has been labeled with a stable isotope.
A method for synthesizing a protein of interest wherein aspartic acid is selectively labeled, which is characterized in that a protein of interest is synthesized in a cell-free protein synthetic system containing a wheat germ extract, under a condition whereby conversion of aspartic acid to glutamic acid is inhibited while the protein synthesis is not inhibited, by using a substrate wherein aspartic acid has been labeled with a stable isotope.
A method for synthesizing a protein of interest wherein glutamic acid is selectively labeled, which is characterized in that a protein of interest is synthesized in a cell-free protein synthetic system containing a wheat germ extract, under a condition whereby conversion of glutamic acid to aspartic acid and/or glutamine is inhibited while the protein synthesis is not inhibited, by using a substrate wherein glutamic acid has been labeled with a stable isotope.
A reagent kit for synthesizing a protein of interest wherein alanine is selectively labeled using a wheat germ extract, which comprises at least a reagent for inhibiting conversion of alanine to aspartic acid and/or glutamic acid.
A reagent kit for synthesizing a protein of interest wherein aspartic acid is selectively labeled using a wheat germ extract, which comprises at least a reagent for inhibiting conversion of aspartic acid to glutamic acid.
A reagent kit for synthesizing a protein of interest wherein glutamic acid is selectively labeled using a wheat germ extract, which comprises at least a reagent for inhibiting conversion of glutamic acid to aspartic acid and/or glutamine.
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