Method for large-scale production of polypeptide in eukaryote cells and a culture vessel suitable therefor
AssigneeNovo Nordisk HealthCare A/G
InventorIda Knudsen
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Abstract
The invention provides a method for large-scale production of a polypeptide, such as a Factor VII or Factor VIIa polypeptide, in eukaryote cells, such as mammalian cells, contained in a culture liquid, said method comprising: monitoring the concentration of dissolved CO2 in the culture liquid, and constantly or intermittently sparging atmospheric air through the culture liquid, wherein the sparging rate of the air is controlled in relation to the monitored concentration of dissolved CO2 in the culture liquid. The method reduces or eliminates the use of bases while providing an excellent pH control. The invention also provides a culture vessel suitable for the methods.
Claims19
A method for large-scale production of a polypeptide in eukaryote cells contained in a culture liquid, said method comprising:
- monitoring the concentration of dissolved CO2 in the culture liquid, and constantly or intermittently sparging air through the culture liquid, wherein the sparging rate of the air is controlled in relation to the monitored concentration of dissolved CO2 in the culture liquid.
A method for large-scale production of a polypeptide in eukaryote cells contained in a culture liquid, said method comprising constantly or intermittently sparging air through the culture liquid at a rate sufficient for maintaining the concentration of dissolved CO2 in the culture liquid within a predetermined range comprising a set-point for said concentration, wherein any solid or liquid substances added to the culture liquid does not give rise to a localized pH value of above 7.5 in said culture liquid.
A culture vessel (1) comprising a culture liquid (2), said vessel being adapted for industrial-scale production of a polypeptide in a eukaryote cell comprised in the culture liquid (2), said vessel further comprising first sensor means (3) for monitoring the concentration of dissolved CO2 in the culture liquid (2), gas sparge means (4) for sparging a gas (such as air) through the culture liquid (2), and control means (5), said control means (5) being in communication with said first sensor means (3), and said control means (5) being in communication with said gas sparge means (4), wherein the control means (5) controls the sparge rate of the gas via the gas sparge means (4) in response to the value of dissolved CO2 monitored by the first sensor means (3).
Dependencies are inferred from the claim text.