Modified Shine-Dalgarno sequences and methods of uses thereof
AssigneeHuman Genome Sciences, Inc.
InventorMichael Laird
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Abstract
Novel Shine-Dalgarno (ribosome binding site) sequences, vectors containing such sequences, and host cells transformed with these vectors are provided. Methods of use of such sequences, vectors, and host cells for the efficient production of proteins and fragments thereof in prokaryotic systems are also provided. In particular embodiments of the invention, compounds and methods for high efficiency production of soluble protein in prokaryotic systems are provided.
Claims18
An isolated polynucleotide comprising a Shine-Dalgarno sequence selected from the group consisting of:
- (a) SEQ ID NO:2;
- (b) polynucleotides 4-13 of SEQ ID NO:2;
- and
- (c) SEQ ID NO:18.
A vector comprising a Shine-Dalgarno sequence selected from the group consisting of:
- (a) SEQ ID NO:2;
- (b) polynucleotides 4-13 of SEQ ID NO:2;
- and
- (c) SEQ ID NO:18.
Dependencies are inferred from the claim text.