Polypeptide factor from a thermophilic eubacterial species and use thereof in the production of functional, heterologous proteins in an expression host
OwnerJoseph Pembroke, Stefania Spada +1inventors
InventorsJoseph Pembroke, Stefania Spada, John Wall
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Abstract
A polypeptide factor derived from the thermophilic eubacterial species Thermus thermophilus has universal protein expression-assisting activity. The polypeptide factor has been named the CzrB protein active in full length or truncated form has the potential to act as a universal protein expression-assisting molecule which can increase the yields of all heterologous proteins produced in E. coli by a mechanism that is independent of the protein being expressed.
Claims6
E. coli bacteria comprising a polypeptide factor from a thermophilic eubacterial species, wherein said polypeptide factor is a metal ion efflux protein, which assists in the expression of heterologous proteins and which confers on E. coli increased resistance to heavy metal ions compared to wild-type E-coli that do not contain the polypeptide factor.
Dependencies are inferred from the claim text.