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US 2006/0216775 A1Published application
Compositions and methods for analysis and manipulation of enzymes in biosynthetic proteomes
AssigneeThe Regents of the University of Califoenia
InventorsMichael Burkart, James Laclair
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Abstract
This invention generally relates to methods and compositions for identifying a protein of interest. The compositions further provide microarrays. The methods provide a viable screen for genetic and proteornic events in natural and engineered systems.
Claims76
A method for detecting a protein of interest comprising:
- contacting a coenzyme with a synthetic appendage label,
- contacting a carrier protein domain with the protein of interest to form a carrier protein (CP) domain-protein of interest (POI) complex,
- contacting the carrier protein (CP) domain-protein of interest (POI) complex with the labeled coenzyme to form a labeled coenzyme-carrier protein (CP) domain-protein of interest (POI) complex, and
- detecting the labeled carrier protein domain to detect the protein of interest.
1 more element
A microarray for identification of a protein of interest (POI) comprising:
- a coenzyme linked to a synthetic appendage label,
- a carrier protein domain contacting the labeled coenzyme and the POI to form a carrier protein-POI-coenzyme complex,
- the synthetic appendage label transferred from the coenzyme to the carrier protein domain within the microarray, wherein the labeled carrier protein domain detects the POI.
Dependencies are inferred from the claim text.