Docetaxel immunoassay
AssigneeSaladax Biomedical Inc.
InventorsSalvatore Salamone, Jodi Courtney, Gregory Lundell, Mahmoud ElSohly, Waseem Gul
Life of this patent
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Abstract
Novel conjugates of docetaxel and novel docetaxel immunogens derived from the 7 and 10 positions of docetaxel and monoclonal antibodies generated by these docetaxel linked immunogens are useful in immunoassays for the quantification and monitoring of docetaxel in biological fluids.
Claims86
An immunoassay for detecting docetaxel in a sample comprising providing a mixture containing a sample, an antibody reactive with docetaxel and a conjugate of a carrier with a ligand selected from a compound of the formula:
- wherein B is
- Y is an organic spacing group;
- X is a functional group capable of binding to a carrier;
- and
- p is an integer from 0 to 1;
- a compound of the formula:
- wherein B, X, Y and p are as above;
- a compound of the formula
- wherein B, X, Y and p are as above,
- and mixtures thereof, causing the docetaxel in the sample and said conjugate to bind with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of docetaxel in the sample can be determined.
An antibody which reacts with docetaxel and does not substantially react with taxol.
A compound of the formula:
- wherein B is
- Y is an organic spacing group;
- X is a functional group capable of binding to a carrier;
- and
- p is an integer from 0 to 1.
A conjugate comprising a carrier linked to a ligand moiety of the formula:
- wherein B is
- Y is an organic spacing group;
- X′ is —CH2—or a functional linking group capable of linking to a carrier;
- and
- p is an integer from 0 to 1.
A compound of the formula:
- wherein B is
- Y is an organic spacing group;
- X is a functional group capable of binding to a carrier;
- and
- p is an integer from 0 to 1.
A compound of the formula:
- wherein B is —CH2—;
- Y is an organic spacing group;
- X is a functional group capable of binding to a carrier;
- and
- p is an integer from 0 to 1.
A conjugate comprising a carrier linked to a ligand moiety of the formula:
- wherein B is
- Y is an organic spacing group;
- X′ is —CH2— or a functional linking group capable of linking to a carrier;
- and
- p is an integer from 0 to 1.
A conjugate comprising a carrier linked to a ligand moiety of the formula:
- wherein B is —CH2—;
- Y is an organic spacing group;
- X′ is —CH2— or a functional linking group capable of linking to said carrier;
- and
- p is an integer from 0 to 1.
A kit for determining the presence of docetaxel in a patient sample comprising reagents packed in separate containers, one of the reagents being an antibody reactive with docetaxel and the other reagent being a conjugate of the carrier with a ligand selected from a compound of the formula:
- wherein B is —CH2—;
- Y is an organic spacing group;
- X is a functional group capable of binding to a carrier;
- and
- p is an integer from o to 1;
- a compound of the formula:
- wherein B, X, Y and p are as above;
- a compound of the formula
- wherein B, X, Y and p are as above;
- and mixtures thereof.
Dependencies are inferred from the claim text.